20+ Ion Exchange Chromatography Schematic Diagram Pics. In an ion exchange column, the stationary phase is a resin on which a usually organic coating provides a charged surface. Chromatography is the separation of a mixture of compounds into its individual components based on their the principle of separation is thus by reversible exchange of ions between the target ions present in the sample solution to the ions present on ion exchangers.
High Performance Liquid Chromatography (HPLC) : Principle ... from laboratoryinfo.com
The stationary phase is selected such that it has a. Column efficiency is expressed as the number of theoretical plates per meter chromatography bed (n) or as h (height equivalent to a theoretical plate, hetp), which is the bed length (l) divided by. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids.
A general definition of ion chromatography can be applied as follows.
Ion exchange chromatography is based on the phenomenon of attraction between opposite charges. Appendix 3, extracted from ion exchange chromatography & chromatofocusing, ge healthcare, 2007. Under the guidence of g.shekhar, mr.uttam 26. These steps are illustrated schematically below.
